The Spaniard will not feature for Los Blancos in the semi-final second leg against their arch rivals, despite returning to training this week

Real Madrid midfielder Isco has been left out of Santiago Solari's Copa del Rey semi-final squad to face Barcelona on Wednesday.

Isco returned to full training at the start of the week after recovering from a back injury, but he will play no part in the crucial showdown with Barca this evening.

Solari has opted to leave the Spaniard out of the second-leg clash, with Madrid looking to build on a positive 1-1 draw at the Camp Nou in the first leg on February 6.

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The Blancos boss has used Isco sparingly during his short reign at the helm, amid reports of a fractious relationship between the pair .

Isco has yet to start a single league game under Solari, with speculation swirling that the 26-year-old will leave the club at the end of the season.

Madrid have won eight of their last 10 matches across all competitions, but a 2-1 home defeat to Girona last week stalled their recent progress.

As a consequence of that result, they slipped nine points behind Barca in the Liga title race, but a 2-1 win against Levante on Sunday ensured that confidence was restored before their latest cup fixture.

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Elsewhere, Solari has included Sergio Ramos in his final 20 to face Barca after the Blancos captain missed the trip to Levante due to suspension.

Despite his match-winning cameo during Madrid's last outing, Gareth Bale looks set to remain on the bench, with Vinicius Jr and Lucas Vazquez likely to play in a front three alongside Karim Benzema once again.

Madrid will, however, be without Marcos Llorente, who is still recovering from a groin injury.

Solari's men will be hoping to advance through to the final at Barca's expense and increase their chances of silverware this term, despite a difficult campaign post-Cristiano Ronaldo.

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The Portuguese winger was sold to Juventus last summer and in his absence, Madrid have not carried the same attacking threat, but they are still in the hunt for a fourth consecutive Champions League crown.

Meanwhile, Barca come into the match with a renewed vigour, having beaten Sevilla 4-2 on Saturday to halt a slide which had seen them secure just one win in their previous five matches.

Full Real Madrid squad to face Barcelona: Thibaut Courtois, Keylor Navas, Luca Zidane; Marcelo, Raphael Varane, Sergio Ramos, Dani Carvajal, Nacho, Alvaro Odriozola, Sergio Reguilon; Toni Kroos, Luka Modic, Casemiro, Federico Valverde, Marco Asensio, Dani Ceballos; Vinicius Jr, Lucas Vazquez, Gareth Bale, Karim Benzema.